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. 2013 Dec 20;8(12):e83429. doi: 10.1371/journal.pone.0083429

Figure 2. Effect of hydrogen on goblet cell hyperplasia of the bronchial epithelium detected by AB/PAS-staining.

Figure 2

(A) Representative goblet cell staining determined by AB/PAS. Con, sham control group; CS, cigarette smoke group; CS+H, hydrogen-rich saline pretreatment group; H, hydrogen-rich saline control group. (Scale bars = 100 µm; lower-left insert: Scale bars = 50 µm); (B) Quantification of AB/PAS-positive area in the airway epithelium. AB/PAS-staining area and total area of corresponding bronchiolar epithelial were measured. AB/PAS-positive rates were presented as the ratio of AB/PAS-positive area to the total area. The values were expressed as mean ± SD from all the rats in each group.*p<0.05, **p<0.01 vs. Control, # p<0.05 vs. CS group.