Figure 3. The superior protocerebrum exhibits differential sNPFR immunoreactive neurons among subcastes (anterior brain view).

A: Nomarsky image of a minor worker brain showing the location of clusters c7 (center) and c2 (lateral). In majors, clusters c7 (B, center), and c2 (C, right; D, left) were not detected. E: shows c7 in the brain of a medium worker (detail in F); however, cluster c2 normally under the lCa is not observed (G, left; I, right). In minors, c7 is clearly visible close to the mCa (H, detail in K) and c2 is located under the right (L) and left lCa (J). The lack of fluorescent signal on the left lCa of a minor worker brain treated with pre-absorbed antibody as negative control is shown in (M), and both brain hemispheres are shown in (N). No fluorescent signal was observed for the pre-immune negative control (O). mCa: median calyces, lCa: lateral calyces, smP: superior medial protocerebrum. Left or right refers to the brain hemisphere shown.