Figure 4. Cluster c5 is immunolocalized at the superior edge of the antennal lobe in all subcastes.

Cluster c5 could be observed in the brain of majors (A), medium (B) and minor (C) workers more clearly from the anterior view but also from the posterior view, slightly deeper in the brain. A schematic representation is shown over a Nomarsky image of a medium worker brain from the anterior view in D. Cluster c5 is most often represented by two cells, which are symmetrically located at the superior edge of the ALo. In the posterior view of the brain in E, c5 is depicted using checkered dots; purple solid dots correspond to cluster c9. Cluster c5 in majors is shown in detail in F (left), G (right); notice that in those images c5 is composed of three cells. H (left), I (right) show a detail of c5 in mediums, and the same is shown in J (left), K (right) for minors. No fluorescent signal was observed in negative controls with pre-immune serum (L) or with the antigen-preabsorbed antibody (M). Left or right refers to the brain hemisphere shown.