Figure 8.

AR79 increases the proportion of ALDH⁺CD133⁺ cells in C4-2B^luc and DU145^luc cell lines. C4-2B^luc and DU145^luc (5×10⁶) were seeded into 10mm plates and treated with indicated concentrations of AR79 or vehicle (DMSO) for 48 hours. Cells were dissociated with Accutase and flow cytometric analysis was performed for ALDH and CD133. A. Flow cytometric analysis showing ALDH and CD133 expression in C4-2B^luc. B. Flow cytometric analysis showing ALDH and CD133 expression in DU145^luc. C and D. Proportion of ALDH⁺;CD133⁺ in C4-2B^luc and DU145^luc, respectively. Data are shown as mean±SD from two independent experiments. *P<0.05 versus vehicle.