Figure 4. CD4+CD164+ demonstrate the phenotype of malignant Sézary cells.

Assessment of cellular morphology, (a-b): CD4+CD164+ cells (5×10⁶, 85% purity) and CD4+CD164− cells (2.9×10⁶, purity 69%) from a high tumor burden patient were recovered using an anti-PE column. Cells were processed to obtain a 1 μm thick section, stained with H&E and assessed for the presence of malignant cells. Cellular morphology was examined using an Olympus BX51 microscope. Images were captured with a Leica DFC 420 camera. Data shown are from one representative patient out of five. Size assessment, (c-d): CD4 T cells from a high tumor burden patient were sorted into CD4+CD164+ and CD4+CD164− (purity of both populations 98%), placed on glass slides, air dried, stained with H&E and analyzed using a Zeiss Axiophot microscope. Images were captured using a Leica DFC 450 camera. Shown images are from one representative patient out of six. Bars =100 μm.