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. 2013 Dec 20;8(12):e84664. doi: 10.1371/journal.pone.0084664

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Map of pRN1 showing native genes. (B) Map of pRN1-based shuttle vector(s) showing the 232-bp putative pRN1 origin of replication (red double arrow). Each vector harbors the pUC19 Ap^r gene and origin (red box), pyrE with or without lacS and a segment of pRN1 highlighted by black arrows. The lacS gene was deleted from pRSP1, 3, and 5 to generate pRSP2, 4, and 6.