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. 2013 Dec 20;8(12):e84664. doi: 10.1371/journal.pone.0084664

Figure 2. Strategy of mapping pRN1 putative origin of replication (double red arrow in Figure 1).

Figure 2

(A) Various deletions were made within the ‘putative ori’ in pRSP1 to generate plasmids highlighted in each rectangular box to determine the role of the deleted regions in pRN1 replication. We constructed pRSP1-NO and pRSP3-NO by deleting 232-bp and 207-bp, respectively, from the putative origin. (B) Three-nucleotide deletion mutants of pRSP1 were constructed to map the loop of stem-loop structure within pRN1 origin. Each construct was transformed into S. acidocaldarius and scored for production of LacS expressing transformants.