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. 2013 Dec 20;8(12):e84664. doi: 10.1371/journal.pone.0084664

Figure 3. Extraction plasmids from S. acidocaldarius transformants and re-transformation into E. coli.

Figure 3

(A) pRN1-based shuttle vectors were recovered from S. acidocaldarius transformants and re-transformed into E. coli to verify that the plasmids were self-replicating. The identity of the plasmids was confirmed by their EcoRI digestion finger-print. (A) EcoRI digestion finger-print of pRSP1, 5 and 7 recovered from (A) S. acidocaldarius E4-39 transformants and (B) E. coli re-transformed with plasmid DNA from the S. acidocaldarius transformants analyzed in (A).