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. 2013 Dec 20;8(12):e84664. doi: 10.1371/journal.pone.0084664

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) The secondary structure of 232-bp putative origin of replication determined using DNA mFold, revealed the presence of 100-bp (4734 - 4833) and 19-bp (4693 - 4711) stem-loop (SL) structures at 70°C. (B) DNA sequence of the 232-bp putative origin of replication showing the 100-bp SL (single underline) and 19-bp SL (double underline) in pRN1 origin. Deletions made in pRSP1a (between ∆), pRSP1b (between∇), pRSP1c (between ▼) and pRSP1d (between ▲) are highlighted by the triangles, while the deletion in pRSP1e is shaded. The ‘GTG’ motif (box) and the loop of the 100-bp SL (red) are also highlighted.