Table 1. Description of plasmids and strains.
|
Plasmid/Strains
|
Description of important features* | Source/ Reference | ||
|---|---|---|---|---|
| Plasmids | ||||
| pUC19 | pMB1 origin, AmpR, (2.7 kb) | |||
| Pc | pRN1 (entire plasmid); pBluescript; pyrEF (9.0 kb) | 3 | ||
| pRSP1 | pRN1 (orfs56/904), AmpR, pyrE and lacS (7.2 kb) | This study | ||
| pRSP1-NO | 4681 - 4912 deleted from pRSP1 (6.9 bp) | This study | ||
| pRSP1a | 4853 - 4912 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1b | 4793 - 4852 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1c | 4733 - 4792 deleted from pRSP1 (7.1 kb)) | This study | ||
| pRSP1d | 4681 - 4732 deleted from pRSP1 (7.1 kb)kb) | This study | ||
| pRSP1e | 4844 - 4855 deleted from pRSP1 (7.2 kb)) | This study | ||
| pRSP1-L1 | 4793 - 4812 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1-L2 | 4773 - 4792 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1-L3 | 4755 - 4772 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1-L4 | 4755 - 4812 deleted from pRSP1 (7.1 kb) | This study | ||
| pRSP1-G1 | 4773 - 4775 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G2 | 4776 - 4778 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G3 | 4779 - 4781 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G4 | 4782 - 4784 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G5 | 4785 - 4787 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G6 | 4788 - 4790 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G7 | 4791 - 4793 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-G8 | 4794 - 4796 deleted from pRSP1 (7.2 kb) | This study | ||
| pRSP1-A1 | A→G substitution at position 4784 | This study | ||
| pRSP1-A2 | A→G substitution at position 4786 | This study | ||
| pRSP1-A3 | A→G substitution at position 4787 | This study | ||
| pRSP1-A7 | A→G substitution at position 4794 | This study | ||
| pRSP1-CL | pRSP1with XhoI-AatII-NruI-PacI-PstI-PvuII-NsiI site (7.2 kb) | This study | ||
| pRSP2 | lacS deleted from pRSP1(5.7 kb) | This study | ||
| pRSP2-CL | pRSP2 with HindIII-XhoI-AatII-NruI-PacI-PstI- PvuII-NsiI sites (5.7 kb) | This study | ||
| pRSP3 | pRN1 (orfs56/904), AmpR, pyrE and lacS (7.6 kb) | This study | ||
| pRSP3-NO | 4707 - 4912 deleted from pRSP3 (7.4 kb) | This study | ||
| pRSP4 | lacS deleted from pRSP3 (6.1 kb) | This study | ||
| pRSP5 | pRN1 (orfs56/904/80), AmpR, pyrE and lacS (8.1 kb) | This study | ||
| pRSP6 | lacS deleted from pRSP5 (6.6 kb) | This study | ||
| pRSP7 | pRN1 (orfs56/904/80/90a/72), AmpR, pyrE and lacS (8.8 kb) | This study | ||
| pRSP9 | orf904 (1986 - 4680) deleted from pRSP1(4.5 kb) | This study | ||
| pRSP10 | orf56 (1815 - 1965) deleted from pRSP1 (7.0 kb) | This study | ||
| pRSP10b | Portion of orf56 (1815 - 1890) deleted from pRSP1 (7.1 kb) | This study | ||
| pGlcSTUV_ko | Source of pyrE-lacS cassette (6.9 kb) | Unpublished | ||
| Strains | ||||
| GX3 | S. acidocaldarius (DSMZ 639) utilizes glucose and galactose) | 14 | ||
| E4-39 | Uracil auxotroph with 17 bp duplication in pyre | This study | ||
| E4-RSP1 | Strain E4-39 harboring pRSP1 | This study | ||
| E4-RSP1-CL | Strain E4-39 harboring pRSP1-CL | This study | ||
| E4-RSP2 | Strain E4-39 harboring pRSP2 | This study | ||
| E4-RSP2-CL | Strain E4-39 harboring pRSP2-CL | This study | ||
| E4-RSP3 | Strain E4-39 harboring pRSP3 | This study | ||
| E4-RSP4 | Strain E4-39 harboring pRSP4 | This study | ||
| E4-RSP5 | Strain E4-39 harboring pRSP5 | This study | ||
| E4-RSP6 | Strain E4-39 harboring pRSP6 | This study | ||
| E4-RSP7 | Strain E4-39 harboring pRSP7 | This study | ||
* pyrE, pyrF and lacS are from S. solfataricus P2; AmpR gene obtained from pUC19