. 2013 Dec 20;8(12):e84664. doi: 10.1371/journal.pone.0084664

Table 2. Transformation efficiency of constructed shuttle vectors into S. acidocaldarius E4-39.

Plasmid(s)	pRN1 fragment	pRN1 fragment location on shuttle vector	Length of pRN1 fragment	Transformation yield (cfu/ng) ^a , ^b
pRSP1	2101 - 5270	1751 - 4920	3170	7.7 × 10³
pRSP2	2101 - 5270	1751 - 4920	3170	4.9× 10³ ^*
pRSP3	2101 - 314	1751 - 5314	3564	3.3 × 10³
pRSP4	2101 - 314	1751 - 5314	3564	4.0 × 10³ ^*
pRSP5	2101 - 814	1751 - 5814	4064	1.3 × 10³
pRSP6	2101 - 814	1751 - 5814	4064	2.2 × 10⁴ ^*
pRSP7	2101 - 1614	1751 - 6614	4864	3.5 × 10²

^a Most transformation experiments were carried out at least two time and efficiency varied with experiments

^b Negative control (without plasmid DNA) was always included with each set of experiments

^* Transformants were classified as small to medium colonies