Table 3. Effect of deletion within putative origin of pRSP1 and pRSP3 on transformation efficiency.
| Plasmid(s) | Regions deleted | Length | Affected feature | Transformation yield (cfu/ng) a , b |
|---|---|---|---|---|
| pRSP1 | Nil | Nil | Putative origin in pRSP1 | 4.8 - 8.4 × 103 |
| pRSP1a | 4853 - 4912 | 60 | 4.8 – 6.6 × 103c | |
| pRSP1b | 4793 - 4852 | 60 | Nil | |
| pRSP1c | 4733 - 4792 | 60 | Nil | |
| pRSP1d | 4681 - 4732 | 52 | 1.5 × 103 – 1.0 × 104c | |
| pRSP1e | 4844 - 4855 | 12 | 5.2 × 103 – 1.0 × 104 | |
| pRSP1-NO | 4681 - 4912 | 232 | Nil | |
| pRSP1-L1 | 4793 - 4812 | 20 | 100-bp stem-loop | Nil |
| pRSP1-L2 | 4773 - 4792 | 20 | Nil | |
| pRSP1-L3 | 4755 - 4772 | 20 | Nil | |
| pRSP1-L4 | 4755 - 4812 | 20 | Nil | |
| pRSP1-G1 | 4773 - 4775 | 3 | Loop of 100-bp stem-loop | Nil |
| pRSP1-G2 | 4776 - 4778 | 3 | Nil | |
| pRSP1-G3 | 4779 - 4781 | 3 | Nil | |
| pRSP1-G4 | 4782 - 4784 | 3 | Nil | |
| pRSP1-G5 | 4785 - 4787 | 3 | Nil | |
| pRSP1-G6 | 4788 - 4790 | 3 | Nil | |
| pRSP1-G7 | 4791 - 4793 | 3 | Nil | |
| pRSP1-G8 | 4794 - 4796 | 3 | Nil | |
| pRSP3 | Nil | Nil | Putative origin in pRSP3 | 2.2 - 3.2 × 103 |
| pRSP3-NO | 4707 - 4912 | 206 | Nil |
a The transformation experiments were carried out at least three times and the efficiency varied with experiments
b Negative control (without plasmid DNA) and positive control (pRSP1 or pRSP3) reactions were always included with each set of experiments
c Colonies are very tiny compared to transformants from pRSP1