Table 4. Co-transformation of pRSP9 with pRSP10 or pRSP10b.
Plasmid(s) | Deleted region | Length | Gene affected | Transformation yield (cfu/ng) a , b |
---|---|---|---|---|
pRSP9 | 1986 - 4680 | 2695 | orf904 | Nil |
pRSP10 | 1815 - 1965 | 151 | orf56 | Nil |
pRSP10b | 1815 - 1890 | 76 | orf56 | Nil |
pRSP9 + pRSP10 | Nil | |||
pRSP9 + pRSP10b | 8.2 × 10° – 4.9 × 10 |
a The transformation experiments were carried out at least three times. The efficiency varied with experiments
b Negative control (without plasmid DNA) and positive control (pRSP1 or pRSP3) reactions were always included with each set of experiments