Fig. 1.
Ex vivo widefield imaging of mouse tongues. White light and autofluorescence images of unstained mouse tongues are shown in the top two rows, respectively. Fluorescence images acquired after staining the specimens with 2-NBDG and proflavine are shown below. A control specimen that was not exposed to the carcinogen is shown in the left column. Specimens at 11, 14, 21, and 29 weeks after initial carcinogen exposure are shown from left to right. Dysplasia begins at 4 weeks postexposure with over half of the specimens developing dysplasia by 12 weeks. Squamous cell carcinoma (SCC) is seen 18 weeks postexposure with over half of the specimens developing SCC by 24 weeks.28 Scale bar is 5 mm.