Table 1. Features that differentiate strain JS1^T with its closely related species of Anoxybacillus.

Features	1	2	3	4	5	6	7*e	8	9	10	11	12	13	14	15	16
Motility	-	+	+	+	+	+	-	+	+	+	+	+	+	+	-	+
Endospore
Shape	S	E*a	O*b	E*j	E/Cy*d	E/Cy*d	S	E/C*f	S*g	O*h	E*i	S*g	E*j	S*k	ND	E*m
Position	T/C	T*a	T*b	T*j	T*d	T*d	T	T*f	T*g	T*h	T*i	T*g	T/ST*j	T*k	ND	C/ST*m
Swelling of sporangia	Slightly	ND	Slightly	+*j	ND	ND	-	ND	ND	ND	-	ND	Slightly*j	ND	ND	+*m
Biochemical tests
Catalase	+	+	+	+	+	+	-	+	+	-	+	+	+	+	+	-
Oxidase	+	+	+	+	+	+	ND	-	+	-	+	+	-	+	+	-
Nitrate reduction	-	-	-	+	-	+	+	-	+	-	+	+	+	-	+	+
Methyl red	+	-	+	-	+	+	ND	-	-	-	-	-	+	-	-	+
Growth characteristics
Temperature range (°C)	40-60	30-66	30-75	30-72	35-70	30-70	37-65	55–67	30-70	37-66	37-69	40-70	37- 60	40-70	30-64	35-70
Temperature optima (°C)	55	60	60	60-65	55	50	62	65	50	57-62	60	50-55	50	55-60	54	60
pH range	5.5-11.5	5.5-10*a	5.0-10.8*b	5.5-9*c	7-11*d	7-11*d	8-10.5	6-7.5*f	6-11*g	5.7-9.9*h	5.5-9.5*i	6-10.5*g	4-9.5*j	6-10*k	7-8*l	6-10*m
pH optima	7.5	7-7.5*a	8*b	7*c	8*d	8.5*d	9.5-9.7	7.2*f	7.5-8.5*g	6.8-8.5*h	8-9*i	7.5-8.5*g	7*j	7.5-8*k	ND	7*m
NaCl tolerance (%)	3.5	3.5	5	2.5	3	4	3	2.5	2.5	3	4.5	4	5	4	3	4
Ethanol tolerance (%)	2	12	2	2	2	4	ND	2	2	-	2	4	-	2	-	3
Hydrolysis of
Gelatin	+	-	+	-	+	+	-	-	+	-	+	-	+	+	-	+
Starch	+	-	+	+	+	+	+	-	+	+	+	+	+	+	-	-
Esculin	+	+	-	+	+	+	ND	+	+	+	+	+	-	-	+	+
DNA	+	+	-	-	-	-	ND	-	+	-	+	+	-	+	+	+
Acid production from D-glucose	+	-	+	+	+	+	ND	-	+	+	+	+	+	+	+	+
Utilization of
Glucose	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+
Raffinose	-	w	w	-	-	-	-	-	-	-	-	+	+	+	+
Mannose	+	w	w	+	+	+	-	+	+	+	+	+	+	w	+
Mannitol	+	+	+	+	+	+	-	-	+	+	+	+	+	+	+	+
Lactose	+	-	-	-	-	-	-	-	-		-	-	-	-	-
Galactose	-	+	+	+	-	+	-	-	+	+	+	+	+	+	+
Xylose	-	+	+	-	-	-	-	-	+	-	-	-	+	+	+	+
Rhamnose	-	-	-	-	-	w	-	w	-	w	+	-	-	-	-	+
Maltose	+	+	+	+	+	+	ND	+	+	-	+	+	+	+	+
Arabinose	-	+	-	w	-	-	ND	-	+	w	-	-	+	w	+
G+C content (mol%)	42.1	42.3*a	44.2*b	41.6*c	42.6*d	41.4*d	42.2+/- 0.2	53.5*f	54*g	42.3*h	45.1*i	50*g	44.4*j	57*k	43.9*l	42.9*m

All data are from present study unless indicated.

Strains: 1, A. suryakundensis strain JS1^T; 2, A. flavithermus subsp. yunnanensis DSM 23293^T; 3, A. mongoliensis DSM 19169^T; 4, A. flavithermus subsp. flavithermus DSM 2614^T; 5, A. eryuanensis KCTC 13720^T; 6, A. tengchongensis KCTC 13721^T; 7, A. pushchinoensis DSM 12423^T; 8, A. thermarum DSM 17141^T; 9, A. ayderensis NCIMB 13972^T; 10, A. kamchatkensis DSM 14988^T; 11, A. salavatliensis DSM 22626^T; 12, A. kestanbolensis NCIMB 13971^T; 13, A. contaminans DSM 15866^T; 14, A. gonensis NCIMB 13933^T; 15, A. voinovskiensis DSM 17075^T; 16, A. kaynarcensis LMG 25303^T.

Data from *^a, Dai et al. [10]; *^b, Namsaraev et al. [13]; *^c, Heinen et al. [3]; *^d, Zhang et al. [12]; *^e, Pikuta et al. [1]; *^f, Poli et al. [8]; *^g, Dulger et al. [5]; *^h, Kevbrin et al. [9]; *ⁱ, Cihan et al. [11]; *^j, De Clerck et al. [6]; *^k, Belduz et al. [4]; *^l, Yumoto et al. [7]; *^m, Inan et al. [14]

S, spherical; E, ellipsoidal; O, oval; Cy, cylindrical; ST, subterminal; C, central; +, positive reaction; w, weakly positive reaction; -, negative reaction; ND, not determined.