Table 3. Selected microbial counts (log cfu/ml digesta) in ileal digesta determined by DAPI staining and fluorescent in situ hybridization (FISH).
| PC | NC | NI100 | NI300 | NI900 | NI2700 | SEM | P | |
|---|---|---|---|---|---|---|---|---|
| DAPI | 9.94c | 10.01ab | 10.00b | 10.04a | 10.01ab | 10.00b | 0.01 | <0.001 |
| Bacteroides-Prevotella | 7.87c | 8.19a | 8.13ab | 7.96bc | 7.85c | 7.82c | 0.13 | <0.001 |
| Clostridium perfringens | 7.77 | 7.71 | 7.87 | 7.82 | 7.82 | 7.70 | 0.13 | 0.369 |
| Enterobacteriacae | 8.01b | 8.27a | 7.93bc | 7.76c | 7.97bc | 7.96bc | 0.16 | 0.002 |
| Lactobacillus/Enterococcus | 8.03 | 8.15 | 8.25 | 8.21 | 8.05 | 8.07 | 0.12 | 0.076 |
PC - positive control (salinomycin, 60 mg/kg); NC - negative control (any additives);NI100 - 100 IU nisin/g (3.2 ml/kg); NI300 - 300 IU nisin/g (9.7 ml/kg); NI900 - 900 IU nisin/g (20.1 ml/kg); NI2700 - 2700 IU nisin/g (87.2 ml/kg)
DAPI - total number of bacteria determined by 4',6-diamidino-2-phenylindole staining
SEM - standard error of the mean
Within the same row, different superscripts indicate significant differences between treatments (P≤ 0.05)