Figure 2.
In 9-day old mice, impaired functional interaction between CBP and CREB leads to reduction in CRH mRNA expression in the paraventricular nucleus (PVN) of the hypothalamus. A, Representative bright-field photomicrographs of coronal sections at the level of the PVN from Cbp+/+, CbpKIX/+ and CbpKIX/KIX mice, subjected to ISH for CRH mRNA. Slides were exposed to film for 10 days. B, Quantification of mRNA levels, in reference to Cbp+/+; Cbp+/+ n = 6, CbpKIX/+ n = 4, CbpKIX/KIX n = 3. A, Scale bar = 500 μm.
