Table 1. Inhibitory effect (%) of pyran naphthoquinones on DENV-2 replication.
| Pyran naphthoquinone (μM) | % of Inhibition of DENV-2 Replication (qPCR)* | % of Inhibition of DENV-2 Replication (TCID50)* |
|---|---|---|
| 8c (0.4) | NT | NT |
| 8d (25) | 0 | NP |
| 9b (100) | 99.0 | 99.83 |
| 9c (100) | 99.6 | 99.99 |
| 9d (0.4) | NT | NT |
| 9e (0.4) | NT | NT |
| 9f (0.4) | NT | NT |
| 10a (12.5) | 0 | NP |
| 10b (12.5) | 0 | NP |
| Ribavirin (200) | 78.00 | NT |
* Screening assays were performed with qPCR for quantification of the produced viral progeny in DENV-2 infected Vero cells at the highest non-toxic concentration of the pyran naphthoquinone compounds, followed by confirmation with Tissue Culture Infectious Dose of 50 (TCID50) assays. For those compounds that did not show inhibition in the qPCR assay the TCID50 assay was not performed (NP).
NT- not tested for viral replication due to its high cytotoxicity both in Vero and HepG2 cells.