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. Author manuscript; available in PMC: 2014 Nov 29.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Nov 5;441(4):10.1016/j.bbrc.2013.10.127. doi: 10.1016/j.bbrc.2013.10.127

Figure 4. Overexpression of SERTAD1 enhanced BMP R-SMAD mediated transcription in cardiomyocytes.

Figure 4

(A) NkL-TAg cells were cultured in 24-well plates and were co-transfected with SBE-lux and different doses of the construct expressing SERTAD1. The cells were treated with or without BMP4 (100ng/ml) for 48 hours, followed by luciferase analysis. The luciferase activity from cells that were not transfected with the Sertad1 construct and were not treated with BMP4 was set at 1.0. The data were averaged from three independent experiments. The error bars represent standard deviation. (B) Same as panel A except that the Nkx2.5-lux(epi) reporter construct was used. (C) NkL-TAg cells were transfected with an empty vector or a plasmid expressing SERTAD1 and treated with or without BMP4 (100ng/ml) for 48 hours. Total protein extracts were subjected to western analysis using antibodies as indicated. The overexpression of SERTAD1 upregulated the expression of the three BMP targeted genes. TUBULIN was used as a loading control.