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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2013 Jan 17;54(1):10.1167/iovs.12-10528. doi: 10.1167/iovs.12-10528

Figure 2. Differential inhibition of mutant K12 protein versus wild type by allele-specific siRNAs in cultured cells.

Figure 2

AD293 cells were transfected with wild type or mutant K12-eGFP construct and each of the siRNAs: K12-Arg135Thr-5, -6, -8,-9,-13,-14 at a final concentration of 5 nM. To confirm the highly differential inhibitory effect of K12-Arg135Thr siRNA on mutant K12, western blot analysis was performed. When cells were treated with K12-Arg135Thr-5, the mutant K12-eGFP was almost completely knocked down, while expression of wild type K12 construct treated with the same siRNA showed a negligible reduction compared with NSC4 treatment. Wt = wild type and mut = mutant.