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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2013 Jan 17;54(1):10.1167/iovs.12-10528. doi: 10.1167/iovs.12-10528

Figure 3. Lack of off-target silencing of other keratins by lead inhibitor K12-Arg135Thr-5.

Figure 3

SimplyBlue staining of cytoskeletal protein extracts from HaCaT cells revealed allele specific K12-Arg135Thr-5 siRNA had negligible effect on endogenous keratins expressed in the HaCaT cell line, most notably K14 which is closely related to K12 in terms of sequence conservation. In contrast, a positive control siRNA directed against wild type K6a demonstrated a dramatic decrease in K6a expression. Non-specific control siRNA NSC4 had no effect on keratin protein expression profile.