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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Chem Commun (Camb). 2012 Nov 4;48(85):10.1039/c2cc35042d. doi: 10.1039/c2cc35042d

Fig. 1.

Fig. 1

Monitoring of cell-SELEX and characterization of selected aptamers. (A) Flow cytometry assay to monitor the binding of selected pools and enrichment of aptamers with CEM cells (target) and Ramos cells (control). For target CEM cells, there was an increase in binding ability of the pool as the selection was progressing, whereas there was little enhancement for the control cells. (B) Flow cytometry assay for the binding of the FITC-labeled sequences sga16 and sgc8 obtained from the above selection. (C) Flow cytometry assay to determine the binding affinity of FITC-labeled sga16 to CEM cells. The nonspecific binding was measured using an FITC-labeled random DNA sequence library. Adapted from ref.14 (Copyright (2006) National Academy of Sciences, U.S.A.)