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. Author manuscript; available in PMC: 2013 Dec 27.
Published in final edited form as: Nature. 2013 Jun 5;498(7455):502–505. doi: 10.1038/nature12216

Fig. 1. Binding between recombinant PfEMP1 and EPCR.

Fig. 1

a) Annotation of proteins (coloured boxes) and their ability to bind EPCR by ELISA (+/−).

b) EPCR binding (ELISA OD, ±s.d.) of 30 recombinant PfEMP1 proteins.

c) SPR sensorgrams of PfEMP1 binding to EPCR and CD36. Binding was tested in two-fold dilutions of PfEMP1 from 125nM for rIT4VAR20, 0.5μM for DD2VAR32 and DD2VAR01 and 1μM for the rest. Green: protein infusion.

d) Sensorgrams for two chips coated with EPCR. Red: EPCR coated chip sequentially flushed with DC8 CIDRα1.1 (2μM), buffer, APC (2μM) and buffer. Blue: EPCR coated chip flushed with APC only.