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. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: ACS Chem Biol. 2013 Oct 7;8(12):10.1021/cb4004089. doi: 10.1021/cb4004089

Figure 4. B1-mediated nuclear delivery of Cre recombinase.

Figure 4

(A) BSR.LNL.td.Tomato cells were exposed to 2.5 μM of Cre-L-B1, Cre-L-R10 or +36GFP-L-Cre at 37°C/5% CO2 for 6 hours. The expression of tdTomato was visualized by fluorescence microscopy 24 hours later. (B) BSR.LNL.td.Tomato cells were exposed to Cre-L-B1 or Cre-L-R10 in the absence or presence of chloroquine (100 μM) at 37°C/5% CO2 for 6 hours. The percentage of cells expressing tdTomato was determined via flow cytometry 24 hours later. The error bars represent the standard deviation of two independent experiments.