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. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: ACS Chem Biol. 2013 Oct 7;8(12):10.1021/cb4004089. doi: 10.1021/cb4004089

Figure 5. B1-mediated cytosolic delivery of RNA and nuclear delivery of DNA.

Figure 5

(A) Gel-shift assays of GFP-L-B1-conjugated DNA and RNA. pIRF RNA (43 femtomoles) or linearized pCMV5 DNA (14.5 femto moles) were incubated with B1 protein at the indicated molar ratios in EMSA buffer for 10 minutes at room temperature analyzed on an agarose gel. (B) 293T cells were transfected with 1.6 nM of pIRF mRNA or linearized pCMV5-Gluc plasmid DNA using GFP-L-B1 (1.6 μM), +36GFP (1.6 μM) or the indicated commercial reagents according to the manufacturer’s directions. The dotted line indicates the limit of detection of the Fluc activity assay. TransMess: TransMessenger. Lipofec: Lipofectamine 2000. Error bars represent the standard deviation from two independent experiments.