Figure 7. B1-mediated cell entry/trafficking utilizes cell-surface glycans.

(A) 293T or TZM-bl cells were incubated with 80 mM of the glycan synthesis inhibitor sodium chlorate (NaClO₃) for 24 hours at 37°C/5% CO₂. GFP-L-B1 (2 μM) was then added to the wells. 1 hour later, the cells were washed with DPBS containing 0.04% Trypan Blue and analyzed via flow cytometry. Representative histograms from two independent experiments are shown. (B) 293T cells were exposed to 80 mM NaClO₃ for 24 hours and then incubated with a mixture containing 5 ng (0.1 nM) pIRF mRNA and 1.6 μM GFP-L-B1 for 6 hours in the presence of NaClO₃. The cells were lysed 6 hours later and the intracellular Fluc signal was measured. The error bars represent the standard deviation of two independent experiments.