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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Bone Miner Res. 2014 Jan;29(1):10.1002/jbmr.2031. doi: 10.1002/jbmr.2031

Figure 3. Strain induces mTORC2 association with FA proteins, dependent on mTOR activity.

Figure 3

a. MSC cell lysates were immunoprecipitated with antibody to vinculin 10 min post-strain and analyzed by immunoblot, normalized to vinculin. Strain increased the association of rictor, mTOR and total and phosphorylated Akt with vinculin. Densitometry of rictor and total Akt bands is shown at the right; * = p < 0.05 different from control, n=3. b. Lysates prior to vinculin immunoprecipitation showed equivalent amounts of proteins of interest. c. Paxillin immunoprecipitation of strained cells showed that association with vinculin, rictor, mTOR and Akt was increased by strain. Densitometry of rictor and Akt bands is shown to the right; * = p < 0.05 different from control, n=3. d. IP with vinculin shows that strain failed in increase the association of rictor and Akt with vinculin when mTOR was inhibited with KU63794 (2mM). Densitometry confirms mTOR activity is necessary for association of rictor and Akt bands with vinculin; * = p < 0.05 different from control, n=3.