Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Bone Miner Res. 2014 Jan;29(1):10.1002/jbmr.2031. doi: 10.1002/jbmr.2031

Figure 6. Insulin activation of Akt induces neither cytoskeletal reorganization nor mTORC2 association with vinculin.

Figure 6

a. Insulin (100 ng/ml x 1 h) does not induce cytoskeletal reorganization, as does 100 cycles of strain, shown by vinculin (red) and actin (green) staining. Scale bars = 25 µm. b. Vinculin pull down after strain (str) or insulin (Ins): insulin does not induce rictor, mTOR or Akt association with vinculin as does strain. Densitometry of rictor and total Akt bands is shown at the right; * = p < 0.05 different from control, n=3. c. siRNA targeting vinculin failed to prevented insulin activation of Akt, shown as an increase phosphor-473 Akt.