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. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: ACS Chem Biol. 2013 Sep 30;8(12):10.1021/cb400592n. doi: 10.1021/cb400592n

Figure 5.

Figure 5

Effects of a series of quinolones and quinazolinediones based on the C7 substituent of 8-Methyl-3′-(AM)P-dione on the DNA cleavage activity of B. anthracis topoisomerase IV. The ability of wild-type (WT, black circles), GrlAS81F (S81F, blue circles), and GrlAS81Y (S81Y, red circles) topoisomerase IV to cleave negatively supercoiled pBR322 DNA in the presence of quinolones (top panels) or quinazolinediones (bottom panels) containing a C7 3′-(aminomethyl)pyrrolidinyl [3′-(AM)P] group and a C8 hydrogen (left panels), methyl (middle panels), or methoxy (right panels) group is shown. Drug structures are shown in the corresponding panels. Error bars represent the standard deviation of three or more independent experiments.