. Author manuscript; available in PMC: 2014 Sep 1.

Published in final edited form as: J Immunol. 2013 Aug 5;191(5):10.4049/jimmunol.1300971. doi: 10.4049/jimmunol.1300971

Table 1.

Neutralization kinetics and potency of induced MPER-specific serum Abs from immunized 2F5 “complete” (V_H^+/+ × V_L^+/+) KI mice^a

Source of sera	MPER-specific serum Ig IC₅₀ and IC₈₀ concentrations for neutralization of HIV-1 (μg/ml
	IC₅₀	IC₈₀
Immunized 2F5 complete KI mice
Pre-immune	NA^b	NA
Post-immune #1	NA	NA
Post-immune #2	0.33	NA
Post-immune #3	0.17	0.59
Post-immune #4	0.21	0.81
Post-immune #5	0.63	2.56
Post-immune #6	0.48	1.62

MPER 2F5 nominal epitope-specific total Ig concentration values for experimental samples, based on values shown in Fig. 2D (and calculated by quantitative ELISA, as described in Materials and Methods) were used to convert GMT values to corresponding estimated concentrations (μg/ml) of MPER-specific serum Igs required to neutralize HIV-1 in the TZM-b/l assay.

NA=Not Applicable, i.e. no neutralization seen in serum.