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. 2010 Autumn;9(4):429–436.

Table 3.

Brine shrimp (Artemiasalina) lethality bioassay of different partitions of Euphorbia Aellenii

Sample a Dose
(μg/mL)
Number of
shrimps
Number of
survivors
LD 50
(μg/mL)
Standard drug LD 50
(μg/mL)
E2b 1000 30 10 177.06 Etoposide 7.4625
100 30 18
10 30 21
E3 1000 30 19 770.66 Etoposide 7.4625
100 30 23
10 30 26
E4 1000 30 26 5346200 Etoposide 7.4625
100 30 27
10 30 28
E5 1000 30 24 377166.8 Etoposide 7.4625
100 30 26
10 30 28

aChloroform (E2), ethyl acetate (E3), n-butanolic (E4) and aqueous (E5) fractions.

bG (Probability value) = 0.48; No. of replicates: 3; Upper limit (upper toxic concentration) = 1878.68 μg/mL and lower limit (lower toxic concentration) = 42.38 μg/mL; incubation condition = 28 ± 1°C