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. Author manuscript; available in PMC: 2015 Jan 25.
Published in final edited form as: Gene. 2013 Oct 31;534(2):10.1016/j.gene.2013.10.045. doi: 10.1016/j.gene.2013.10.045

Figure 1. Lhx3 mRNA and protein are expressed in mouse pituitary Pit-1/Triple but not Pit-1/0 cells.

Figure 1

(A) Lhx3a or Lhx3b mRNA was amplified by RT-PCR in Pit-1/0 and Pit-0/Triple cells. Beta actin primers were used in a positive control to test cDNA integrity. (B) Protein levels of LHX3 were determined by western blotting of whole cell protein extracts of Pit-1/0 and Pit-1/Triple cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were used as positive controls. Each experiment was performed three times with similar results.