Table 1.

Experimental details used for immunohistochemistry.

Antigen	Period used for	Clone	Type	Antigen retrieval	Source
ER	Until Oct 2002	ID5	Mouse monoclonal	A/C citrate buffer	Dako
	From Nov 2002 to Feb 2005	ER88	Mouse monoclonal	As above	Bio Genex
	From Mar 2005	ID5	Mouse monoclonal	As above	Dako
PgR	Until Oct 2002	IA6	Mouse monoclonal	As above	Novocastra
	From Nov 2002 to Feb 2005	PR88	Mouse monoclonal	As above	Bio Genex
	From Mar 2005	PgR636	Mouse monoclonal	As above	Dako
HER2	Until Oct 2002	c-erbB-2	Rabbit polyclonal	As above	Dako
	From Nov 2002 to Feb 2005	CB11	Mouse monoclonal	As above	Bio Genex
	From Mar 2005	c-erbB-2	Rabbit polyclonal	As above	Dako

A/C: autoclave for 10 min at 121°C; ER: estrogen receptor; HER2: human epidermal receptor 2; PgR: progesterone receptor; citrate buffer: 10 mM citrate buffer, pH 6.0.