Table 1.
Different characteristics between the five HER2 genetic assays.
| Dako HER2 FISH | Dako HER2 IQ-FISH | Dako HER2 CISH | ZytoVision HER2 FISH | ZytoVision HER2 CISH | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 |
| Probe | DNA | PNA | DNA | PNA | DNA | PNA | DNA | DNA | DNA | DNA |
| Label color | TxRed | FITC | TxRed | FITC | Red | Blue | FITC | Rhodamine | Green | Red |
| Blocking reagent | alu-PNA | alu-PNA | alu-PNA | Repeat free | Repeat free | |||||
| Visualization | Fluorescence | Fluorescence | Chromogenic | Fluorescence | Chromogenic | |||||
| Hybridization reagent | Formamide | Ethylene carbonate | Formamide | Formamide | Formamide | |||||
CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization; PNA: peptide nucleic acids.