Table 1.
Survival and observed transgene expression efficiencies from injected DNA
| Construct Injected | Amount (pg) |
Injected Embryos |
Scoreda | Survivalb (%) |
Correct Expressors |
Partialc | Ectopib | GFP- | Correct Expressors (%) |
|---|---|---|---|---|---|---|---|---|---|
| Pax6 BAC | 20 | 216 | 197 | 91% | 127 | 37 | 9 | 24 | 64 |
| Pax6 BAC | 40 | 151 | 87 | 58% | 49 | 5 | 2 | 31 | 56 |
| Pax6 BAC | 80 | 114 | 46 | 40% | 18 | 6 | 3 | 19 | 39 |
| Xath5 BAC | 20 | 91 | 76 | 84% | 43 | 25 | 1 | 7 | 57 |
| Xath5 BAC | 40 | 129 | 33 | 26% | 14 | 5 | 0 | 14 | 42 |
| Xath5 BAC | 80 | 151 | 22 | 15% | 8 | 3 | 1 | 10 | 36 |
| RaxGFPfusion BAC | 20 | 113 | 55 | 49% | 18 | 2 | 7 | 28 | 33 |
| RaxGFPfusion BAC | 40 | 149 | 79 | 53% | 31 | 11 | 5 | 32 | 39 |
| RaxGFPfusion BAC | 80 | 154 | 17 | 11% | 3 | 0 | 2 | 12 | 18 |
| IS Pax6GFP | 20 | 138 | 62 | 45% | 0 | 0 | 54 | 8 | 0 |
| IS Pax6GFP | 40 | 100 | 19 | 19% | 0 | 0 | 18 | 1 | 0 |
| Pax6-GFP pTARBAC2.1 | 40 | 142 | 73 | 51% | 2 | 6 | 14 | 51 | 3 |
| Pax6-GFP pTARBAC2.1 | 80 | 150 | 39 | 26% | 0 | 1 | 9 | 29 | 0 |
a
Only embryos with normal appearance were scored.
b
Indicates the percentage of normal embryos scored from the injected count.
c
Indicates embryos expressing in a subset of correct tissues for a given reporter. IS Pax6GFP-injected and Pax6-GFP pTARBAC2.1-injected embryos were scored as correct expressors if GFP expression recapitulated either endogenous Pax6 expression or previously reported integrated transgene expression (Hirsch et al., 2002; Ogino et al., 2006).