Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 9;65:10.1016/j.freeradbiomed.2013.10.001. doi: 10.1016/j.freeradbiomed.2013.10.001

Figure 2.

Figure 2

Characterization of the initial-velocity data of GR from rat liver [27] and mouse liver [28]. For GR obtained from rat liver studies, the enzyme assays were carried out with 0.3 nM of enzyme at pH 7.6 and 30°C. (A) Model fits to the initial-velocity data with varying [NADPH] and different [GSSG]. (B) Model fits to the effect of product NADP+ on the activity of GR for two different [GSSG] at a fixed [NADPH] (10.7 μM). For GR obtained from mouse liver studies, the enzyme assays were carried out with 2.6 nM of enzyme at pH 7.0 and 25°C. (C) Model fits to the initial-velocity data with varying [NADPH] and different [GSSG]. (D) Model fits to the initial-velocity data with varying [GSSG] and different [NADPH].