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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 9;65:10.1016/j.freeradbiomed.2013.10.001. doi: 10.1016/j.freeradbiomed.2013.10.001

Figure 3.

Figure 3

Characterization of the initial-velocity data of GR purified from human erythrocytes [34]. Here enzyme assays were carried out with 0.6 μM of enzyme for forward studies at pH 7.0 and 25°C. (A) Model fits to the initial-velocity data with varying [NADPH] and different [GSSG]. (B) Model fits to the initial-velocity data with varying [GSSG] and different [NADPH]. (C) Model fits to the effect of product NADP+ on the activity of GR with varying [NADPH] at a fixed 1 mM [GSSG]; the enzyme concentration used here was 0.08 μM. (D) Model fits to the effect of product NADP+ on the activity of GR with varying [GSSG] at a fixed 100 μM [NADPH]; the enzyme concentration used here was 0.1 μM.