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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 9;65:10.1016/j.freeradbiomed.2013.10.001. doi: 10.1016/j.freeradbiomed.2013.10.001

Figure 4.

Figure 4

Characterization of the initial-velocity data of GR purified from bovine liver [33]. For GR obtained from bovine liver the initial-velocity experiments were carried out at pH 7.4 and 37°C. (A) Model fits to the initial-velocity data with varying [GSSG] at different [NADPH]. (B) Model fits to the effect of product NADP+ on the activity of GR with varying [NADPH] at fixed 0.7 mM of [GSSG]. (C) Model fits to the effect of product NADP+ on the activity of GR with varying [GSSG] at fixed 100 μM of [NADPH]. (D) Model fits to the effect of product GSH on the activity of GR with varying [NADPH] at a fixed 0.7 mM [GSSG]. (E) Model fits to the effect of product GSH on the activity of GR with varying [GSSG] at a fixed 100 μM [NADPH]. The estimated enzyme concentrations used in all above experiments were 0.7 nM.