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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 9;65:10.1016/j.freeradbiomed.2013.10.001. doi: 10.1016/j.freeradbiomed.2013.10.001

Figure 5.

Figure 5

Characterization of the initial-velocity data of GR purified from P. blakesleeanus [30]. Here the enzyme assays were carried out at pH 7.5 and 30°C. (A) Model fits to the initial-velocity data with varying [NADPH] and different [GSSG]. (B) Model fits to the initial- velocity data with varying [GSSG] and different [NADPH]. (C) Model fits to the effect of product NADP+ on the activity of GR with varying [NADPH] at a fixed 1 mM [GSSG]. (D) Model fits to the effect of product NADP+ on the activity of GR with varying [GSSG] at a fixed 40 μM [NADPH]. (E) Model fits to the effect of product GSH on the activity of GR with varying [NADPH] at a fixed 1 mM [GSSG]. (F) Model fits to the effect of product GSH on the activity of GR with varying [GSSG] at a fixed 40 μM [NADPH]. The estimated enzyme concentrations used in all above experiments were 0.7 nM.