Figure 6.

Effect of pH on GR activity. (A) Initial-velocity of GR with variable [GSSG] at a fixed 100 μM [NADPH] for three different pH values. Here the experimental conditions are similar to Figure 5. (B) Model simulations for GR activity with varying pH for four different [GSSG] and at fixed [NADPH] (0.1 mM). Here, dashed, dotted, dash-dotted and solid line represent 0.25, 0.5, 1 and 5 mM of [GSSG], respectively.