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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 9;65:10.1016/j.freeradbiomed.2013.10.001. doi: 10.1016/j.freeradbiomed.2013.10.001

Figure 8.

Figure 8

Model simulations of GR kinetics for in vivo-like conditions. The GSH and NADPH pools are set to 10 mM and 0.1 mM, respectively. The % GSSG is calculated as the ratio of its concentration to the total GSH pool concentration (Gtot), which is defined as Gtot = GSH + 2×GSSG. Percentage fluxes of GR with both varying NADPH and GSSG at optimal pH 7.5: (A) total flux, (B) sequential flux, and (C) ping-pong flux. At acidic pH 6.0: (D) total flux, (E) sequential flux, and (F) ping-pong flux. The model was simulated using estimated parameters from Montero et al. [30]. All the total flux values were obtained by dividing with the maximum flux value in the absence of products. Ping-pong and sequential fluxes were calculated as the fraction of the total flux for the respective substrate concentrations.