FIG 3 .

Characterization of 5′ and 3′ extremities of SvsRNA-1. (A) Synthetic oligonucleotides that have 5′ OH or 5′ P ends or total RNA were treated with Terminator exonuclease (+) or not treated with Terminator (−) before being analyzed by Northern blotting. (B) β-elimination on HEK293-SINV total RNA followed by Northern blotting of SvsRNA-1 and miR-16 after migration on 17.5% polyacrylamide gel. Asterisks denote putative precursors of SvsRNA-1. (C) Synthetic SvsRNA-1 molecules that have 3′ P, 3′ OH, or 3′ OH 2′-O-me ends were treated with calf intestinal phosphatase (CIP) (lanes 2, 4, 6, 8, 10, 12, 14, and 16). Dephosphorylation was followed by β-elimination (lanes 4, 8, 12, and 16). oligo, oligonucleotide; me, methyl; elim, elimination.