FIG 1 .

Genetic structure of Tn6215 and site of host chromosome integration. In recipient strain CD062, a putative transcriptional regulator gene was uninterrupted. In donor strain CD80 and transductant strain CD062E1, Tn6215 was integrated into the putative transcriptional regulator gene and the site of integration, TCC, was duplicated at either end. Predicted functional modules of the 13-kb transposon are color coded as follows: red, resistance; yellow, mobilization and replication; green, stability; gray, recombination; and white, indeterminate. Restriction sites are indicated as follows: B, BsrGI; H, Hyp99I; and M, MfeI. Probes used in this study are shown as hatched bars and are shown under their target regions. Homologues in other mobile elements and plasmids are indicated by colored outlines of putative genes as follows: purple, Tn5397; brown, pMV158; blue, CTnBST; pink, CTnDOT; orange, pSM19035; and turquoise, Tn5398. Primers P1 to P7 flanking host and transposon junctions are indicated by black arrows.