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Growth curves of nitrogen-replete (red) and nitrogen-limited (blue) batch cultures of “Ca. Pelagibacter ubique.” Artificial seawater medium contained 500 µM oxaloacetate, 10 µM DMSP, 500 µM pyruvate, and trace nutrients (see Table S1 in the supplemental material). Arrows indicate time points where samples for proteomic and microarray analysis were collected.
