FIG 5 .

C. albicans PKH

kinase mutants do not exhibit an invasive hyphal growth defect. (A) Indicated strains were spotted onto 4% BCS containing 1.5% agar and incubated at 37°C for 7 days. (B) Serial dilutions of the indicated strains were spotted onto YPD agar medium, YPD containing 0.06% SDS, and YPD containing 40 µg/ml Congo red and then incubated at 30°C for 48 h. The pkh1Δ mutant strain carrying only one copy of PKH3 demonstrated greater sensitivity to both cell wall-damaging agents, SDS and Congo red, than either the pkh1Δ or pkh3Δ single mutant strain. Strains used included the wild type, DIC185, and the nce102Δ (YHXW14), pkh1Δ (YLD91-1-9-1), pkh3Δ (YLD95-4-5-2), and pkh1Δ pkh3Δ/PKH3 (YLD146-6-54-1) strains.