FIG 3 .

Deletion of both PadsbB1 and PadsbB2 impairs the oxidative folding of extracellular appendages and exoproteins. (A) Strains lacking PadsbA1 or PadsbB1 and PadsbB2 have twitching (left) and swimming (right) motility defects, but not the single PadsbB1 and PadsbB2 mutants. (B) In order to identify additional exoproteins affected by the impairment of the disulfide formation machinery, we analyzed the culture supernatants prepared from the wild type (WT) and the various Padsb mutants on a Coomassie brilliant blue-stained SDS-polyacrylamide gel (12%). The five proteins that are secreted by the wild type but not by the PadsbA1 and PadsbB1B2 mutants are indicated on the left. The molecular weight markers (in kilodaltons) are indicated on the right.