FIG 7 .

Pet bound to CK8 undergoes clathrin-mediated endocytosis. HEp-2 cells were treated with Pet (37 µg/ml) for 1, 2, 4, or 8 min. Cells were then washed, fixed, and permeabilized. Clathrin was detected using a polyclonal anti-clathrin antibody followed by a secondary anti-goat IgG antibody coupled to fluorescein (green), CK8 was detected using a mouse monoclonal anti-CK8 antibody followed by a secondary antibody anti-mouse IgG coupled to rhodamine (red), and Pet was detected using a rabbit anti-Pet polyclonal antibody followed by a secondary anti-rabbit IgG antibody coupled to Cy5 (blue). Slides were observed by confocal microscopy.