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. 2013 Dec 10;4(6):e00838-13. doi: 10.1128/mBio.00838-13

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Copyright © 2013 Nava-Acosta and Navarro-Garcia.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 8 — Pet and CK8 colocalize as a ligand-receptor complex in early endosomes of epithelial cells. HEp-2 cells were treated overnight with the CellLight early endosomes-RFP BacMam 2.0 reagent to mark Rab5b, a resident protein of early endosomes (red). Cells treated with Pet for 7, 8, 9, or 10 min were then washed, fixed, and permeabilized. CK8 was detected using a mouse monoclonal anti-CK8 antibody followed by a secondary anti-mouse IgG antibody coupled to fluorescein (green), and Pet was detected using a rabbit polyclonal anti-Pet antibody followed by a secondary anti-rabbit IgG antibody coupled to Cy5 (blue). Slides were observed by confocal microscopy.