Figure 1.

DS2 application to cerebellar granule cells enhances the tonic conductance but does not alter IPSC properties. (A) All-point histograms were constructed from 100 ms current traces (V_H −60 mV) recorded from a cerebellar granule cell in control conditions and in the presence of 10 μM DS2 and 500 nM THIP and the solid lines are Gaussian fits to the distributions. (B) The time course plot illustrates the change in average holding current calculated every 1 s from consecutive 100 ms current epoch. An enhancement of the tonic conductance was observed during DS2 and THIP applications. (C) Scatter plot of peak amplitude against decay time for all IPSCs recorded from this granule cell in the presence (filled squares, grey) and absence (filled circles, black) of DS2. The histograms on each axis illustrate Gaussian fits to the distributions constructed in the presence and absence of DS2. (D) Scatter plot of the average tonic conductance and average sIPSC decay before and after DS2 application. The data for individual cells are linked by dashed lines and arrows to illustrate the consistent increase in the tonic conductance that takes place with no consistent change in the IPSC decay. Superimposed on this plot are the average values across all cells (mean ± SEM, n = 5).