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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Cancer Res. 2013 Sep 26;73(22):10.1158/0008-5472.CAN-13-1002. doi: 10.1158/0008-5472.CAN-13-1002

Figure 3.

Figure 3

Chk1 promotes cell viability, clonogenicity and anchorage-independent growth of CIP2A-dependent human cancer cells. A, Inhibition of cell viability by two different Chk1 siRNAs in AGS, B, MKN-28 and C, MDA-MB231 cells. Shown are mean values + S.D., of representative results from three independent experiments (student's t-test). D, Assessment of clonogenic potential of AGS cells when transfected with Scr. (control), Chk1 and Claspin(CLSPN) siRNAs E, Assessment of clonogenic potential of AGS cells stably transfected with Control and two different Chk1 shRNAs, F, Effect of two small molecule inhibitors of Chk1 (1uM) on clonogenic potential of AGS cells. Shown are representative pictures with quantitated colony numbers from that experiment. G, Effect of two different Chk1 shRNAs on anchorage-independent growth of MKN-28 cells in soft agar assay. Representative pictures from each well are shown above the bar graph. Shown is mean values + S.D., of representative results from three independent experiments (student's t-test)